In Article <199410181946.AA21579 at ponyexpress.princeton.edu>,
yuan at PHOENIX.PRINCETON.EDU ("Jeffrey Yuan") wrote:
>I have been told that it is possible to do artificial insemination of
>hermaphrodites with sperm from males that do not mate. However, what I have
>heard is 3rd hand experience. Has anyone done this procedure? If so, I would
>be interested in finding out how. Thanks in advance!
>>Jeffrey Yuan
>>**************************************************************************
>* Jeffrey Yuan *
>* Department of Molecular Biology *
>* Lewis Thomas Laboratory *
>* Princeton University FAX: 609-258-3980 *
>* Washington Road Phone: 609-258-5939 *
>* Princeton, NJ 08544 email: yuan at phoenix.princeton.edu *
>**************************************************************************
>Yes, it is possible. Craig LaMunyon, a post-doc in the Ward Lab has developed
this technique. Look for it (probably) in the November issue of Genetics:
"Assessing the Viability of Mutant and Manipulated Sperm by Artificial
Insemination of Caenorhabditis elegans" by Craig W. LaMunyon and Samuel
Ward. If you need any further (or quicker) information, you can reach Craig
at the phone number or email address below.
--------------------------------------------------------------------------------
Paul J. Muhlrad
Department of Molecular and Cellular Biology
University of Arizona
415 Life Sciences South
Tucson, AZ 85721
tel (602)621-9135
fax (602)621-3709
email Ward_Lab at tikal.biosci.arizona.edu
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