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Letter from Morris Maduro, Injection problems: Reply

Fri Oct 28 07:13:18 EST 1994

Dear Morris,
1. I do not believe that it is lacZ that causes problems.
2. I have tried several preparation methods for DNA to be injected. 
Minipreps which include and RNase treatment have never worked for me,
omitting the RNase helps. Significantly better is a Qiagen prep (obviously,
you get rid of the RNase on the column) or a CsCl prep. Similar results
were obtained by other people in our lab.
These observations are based on 70+ injections.

Good luck

Ralf Baumeister
Ruvkun lab
Mass. General Hospital 

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