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egg permeabilization

Tritech Research Tritech_Research at lamg.com
Fri Dec 22 03:45:29 EST 1995

Dear Chris,

As you may have seen at the last Worm Meeting, we are
working on a hand-held electroporation device called
The Cloning Gun.  The version for E. coli and yeast works
great, but I'm trying to make a version for simplified
C. elegans transformation.  So far we have gotten some
transformants, but not efficiently enough to replace
microinjection yet.

HOWEVER, I can tell you that it permeablizes eggs
(and whole worms) GREAT.  (If you don't mind them
being mostly dead).  If you want more info, e-mail
or call me at (310) 312-0085.

Regards, a wonderful holiday, and happy and healthy 1996 to all wormers,

Andy Papp


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