>I am working to try to fluorescently label the egg cell nuclei of
>Heterodera glycines eggs so that I may evaluate the developmental stage
>of egg populations using flow cytometry.
>>Chris Marett
>cmarett at iastate.edu
Chris,
You didn't say whether it is OK to kill the eggs in the process. I'll
assume it is since you're doing flow cytometry afterward.
I know of two treatments sufficient to permeabilize C. elegans eggs with
high efficiency, though not everything in these protocols may be actually
necessary. They are:
1 hypochlorite treatment (has the advantage of getting rid of all tissues
except eggs) followed by formaldehyde fixation.
2 formaldehyde fixation accompanied by a freeze-thaw cycle.
For more information, check out this site on the web:
gopher://weeds.mgh.harvard.edu:70/00/caenorhabditis/mgh_protocol
There are two protocols there that might be of interest, one on
hypochlorite treatment and one on fixation and permeabilization.
Mike
Michael Finney, PhD | /\/\\_\ /\_\ | 149 Grove Street
MJ Research, Inc. | /\_\_\\ \\_\ | Watertown MA 02172
mikef at mjr.com | /_/ | 617 923 8001 x112
