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egg permeabilization

Michael Finney mikef at mjr.com
Fri Dec 22 18:05:34 EST 1995

>I am working to try to fluorescently label the egg cell nuclei of
>Heterodera glycines eggs so that I may evaluate the developmental stage
>of egg populations using flow cytometry.
>Chris Marett
>cmarett at iastate.edu


You didn't say whether it is OK to kill the eggs in the process. I'll
assume it is since you're doing flow cytometry afterward.

I know of two treatments sufficient to permeabilize C. elegans eggs with
high efficiency, though not everything in these protocols may be actually
necessary. They are:

1 hypochlorite treatment (has the advantage of getting rid of all tissues
except eggs) followed by formaldehyde fixation.

2 formaldehyde fixation accompanied by a freeze-thaw cycle.

For more information, check out this site on the web:


There are two protocols there that might be of interest, one on
hypochlorite treatment and one on fixation and permeabilization.


Michael Finney, PhD |     /\/\\_\ /\_\    | 149 Grove Street
MJ Research, Inc.   |    /\_\_\\ \\_\     | Watertown MA 02172
mikef at mjr.com       |          /_/        | 617 923 8001 x112

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