In article <maske-1007951511370001 at mel.uoregon.edu>,
maske at molbio.uoregon.edu (MILO) wrote:
> Has anyone had any luck growing C. elegans in liquid culture? If so,
> or if anyone has grown up large quantities of worms by another method,
> please let me know. Thanks.
I have had good luck using the following protocol:
1. Grow OP50 to saturation in 2x YT medium.
2. Pellet and resuspend in 1/2 vol of the following medium:
10 ml 1M K Citrate
10 ml trace metals
3 ml 1M CaCl2
3 ml 1M MgSO4
1 ml 10 mg/ml cholesterol
3. Add worms that have been washed off of a uncontaminated plate. I
inoculate a 500 ml culture with worms from a 100 mm plate.
4. Grow with constant shaking at 20-25 C . It should take 4-5 days for
the culture to reach saturation.
I often find it necessary to add an antifungal agent such as nystatin.