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Liquid Culture

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Tue Jul 11 19:21:26 EST 1995

In article <maske-1007951511370001 at mel.uoregon.edu>, 
maske at molbio.uoregon.edu says...
>   Has anyone had any luck growing C. elegans in liquid culture?  If so,
>or if anyone has grown up large quantities of worms by another method,
>please let me know.  Thanks.

I have had a lot of success growing OP50 in liquid NG media overnight at 
22 to 26C at 150 rpm.  The next day inoculating with C. elegans.  I grew 
100 mL cultures and got a lot of worms in 5 days (>1000/mL).  If you are 
interested in large scale, Bio/Techniques had an article about growing 
100 L cultures in the Jan issue of 1994 (or sometime around then).

Good Luck.


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