In article <3v9lli$n77 at news.ycc.yale.edu>, RBURDINE at biomed.med.yale.edu
(R.O.S.B.F.) wrote:
> Hi Netters,
>>>> I really have two questions.
>
> First off, I ahve GFP staining from a constrcut I injected into e1282 using
> MH86 as my injection marker. The staining is intense and I need to get some
> pictures of it obviously. My problem is the staining is COMPLETELY quenched
> by NaAzide.
Try anesthetizing the worms with 1mM levamisole. This was suggested in a
WBG article, probably from the Chalfie or Fire labs.
