Summary of micromanipulators recommended for DNA microinjection:
I asked for recommendations on micromanipulators for C.elegans DNA
microinjection (before I left for vacation two weeks ago). Below are
excertps of the responses I've received so far.
MAIN CONCLUSION:
For my purposes, the main conclusion is that folks have been satisfatied
with relatively simple manipulators, such as Narishige's MN-151 model which
costs about $1500 (before this week's price increase), as opposed to the
more expensive models which can cost thousands more.
EXCERPTS:
David Baillie <dbaillie at darwin.mbb.sfu.ca> writes:
>we use an old Lietz mechanical manipulator
Mike Gruidl <gruidl at medsci.mbp.missouri.edu> writes:
>Here in Karen Bennett's lab we use the Leica manipulator.
Eric.J.Lambie at Dartmouth.EDU (Eric J. Lambie) writes:
>OK, I'll cast my vote: I like the Narishige MN151. It's cheap and attaches
>directly to the stage, but it does an excellent job of positioning the needle
>exactly where you want it - assuming you're one of those people who likes to
>push the worm into the needle, rather than vice versa.
okkema at tigger.cc.uic.edu (Pete Okkema) writes:
> I am also going to purchase a micromanipulator soon. When I was in Andy
>Fire's lab we used a Zeiss-Jena manipulator. Very simple and sturdy, but
>they don't make them any more. You may be able to find one at microscopy
>supply houses for ~$1500-2000.
Gautam Kao <kao at UMDNJ.EDU> writes:
>Both Monica Driscoll's lab and mine get our needle puller and
>micromanipulator from Narishige. We have the same manipulator, model
>MN-151.
Curtis Loer also sent me a summary of comments he received earlier in
response to a similar survey. I've extracted those comments relating to
micromanipulators.
