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SUMMARY: micromanipulators recommended for DNA microinjection

William R. Morgan wmorgan at ACS.WOOSTER.EDU
Mon Jun 26 11:17:53 EST 1995

Summary of micromanipulators recommended for DNA microinjection:

I asked for recommendations on micromanipulators for C.elegans DNA
microinjection (before I left for vacation two weeks ago).  Below are
excertps of the responses I've received so far. 


For my purposes, the main conclusion is that folks have been satisfatied
with relatively simple manipulators, such as Narishige's MN-151 model which
costs about $1500 (before this week's price increase), as opposed to the
more expensive models which can cost thousands more.


David Baillie <dbaillie at darwin.mbb.sfu.ca> writes:
>we use an old Lietz mechanical manipulator

Mike Gruidl <gruidl at medsci.mbp.missouri.edu> writes:
>Here in Karen Bennett's lab we use the Leica manipulator.  

Eric.J.Lambie at Dartmouth.EDU (Eric J. Lambie) writes:
>OK, I'll cast my vote: I like the Narishige MN151.  It's cheap and attaches
>directly to the stage, but it does an excellent job of positioning the needle
>exactly where you want it - assuming you're one of those people who likes to
>push the worm into the needle, rather than vice versa. 

okkema at tigger.cc.uic.edu (Pete Okkema) writes:
>  I am also going to purchase a micromanipulator soon.  When I was in Andy
>Fire's lab we used a Zeiss-Jena manipulator.  Very simple and sturdy, but
>they don't make them any more.  You may be able to find one at microscopy
>supply houses for ~$1500-2000.  

Gautam Kao <kao at UMDNJ.EDU> writes:
>Both Monica Driscoll's lab and mine get our needle puller and 
>micromanipulator from Narishige.  We have the same manipulator, model 

Curtis Loer also sent me a summary of comments he received earlier in
response to a similar survey.  I've extracted those comments relating to

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