When I was sequencing the cDNA clones from Uni-Zap XR library, I found that
several clones share the same small stretch of nucleotides GGCACGAG after
EcoRI site. Theoretically, all the DNA between EcoRI and XhoI is the cDNA
insert. So I am just wondering why there is this strech of nuceotides.
If anybody happened to see this kind of things before or know what
is going on, offer your help please.
Thank you for your attention.
biolc4 at jetson.uh.edu