I am looking for a simple method of staining worms with Hoechst
after b-gal or antibody staining. Can they just be mounted in PBS-Hoechst
or does it have to be washed out? Also has anyone done b-gal, antibody and
DNA staining in the same specimen? Any advice would be welcome.
Thanks,
Terese Rakow <Maruyama lab>
The Scripps Research Institute