In article <950930.072047.EDT.3000JGER at vm1.ulaval.ca> Julie Gervais,
3000JGER at VM1.ULAVAL.CA writes:
>Hi! I am trying to purify the homologue of a yeast protein in C.elegans.
>However, the yeald of protein starting from adult worm culture is low and
>since I tried to sonicate them, proteolysis may be a problem.
There is an article by Gbewonyo et al., (1994) on large scale cultivation
of C. elegans which may be of use to you: Bio/Technology 12:51-54. You
could also try using baffled flasks to increase aeration of liquid
cultures, and hopefully, the worm yield. I use grinding in a liquid
nitrogen-cooled mortar and pestle (1.5-2 ml buffer per gram worm pellet)
to get lysates which yield cytosols of around 8 mg/ml protein.
Ron
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"People who pride themselves on their "complexity" and deride others
for being "simplistic" should realize that the truth is often not
very complicated. What gets complex is evading the truth."
-Thomas Sowell
Syndicated Columnist
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Ron Kagan
rkagan at ewald.mbi.ucla.edu
