In article <44n53u$12ru at saba.info.ucla.edu>, rkagan at ewald.mbi.ucla.edu
says...
>>In article <950930.072047.EDT.3000JGER at vm1.ulaval.ca> Julie Gervais,
>3000JGER at VM1.ULAVAL.CA writes:
>>>Hi! I am trying to purify the homologue of a yeast protein in
C.elegans.
>>However, the yeald of protein starting from adult worm culture is low
and
>>since I tried to sonicate them, proteolysis may be a problem.
>>There is an article by Gbewonyo et al., (1994) on large scale
cultivation
>of C. elegans which may be of use to you: Bio/Technology 12:51-54. You
>could also try using baffled flasks to increase aeration of liquid
>cultures, and hopefully, the worm yield...
>>Ron Kagan
An appreciable amount of C. elegans can be easily grown in liquid
culture. I had a lot of success growing them for bioassays. I simply
took about 100 mL of NG media in a 250 mL flask, inoculated with OP50,
grew overnight and inoculated with N2 worms. After about 5 - 7 days at
150 rpm 24 - 28 C, I had several thousand worms/mL. You may want to try
this with serveral Furnbach flasks. It may be a lot easier than dealing
with fermenters.
Let me know if this helps.
Tim Dvorak
