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help with a biochemistry lab

Ron Tate rtate at bmb-fs1.biochem.okstate.edu
Mon Jun 10 16:15:48 EST 1996

Bruce Wightman wrote:
> I was wondering if anyone could provide suggestions for good proteins to
> partially purify/purify for an introductory biochemistry lab for
> undergraduates.  I'd like to be able to give students an experience with
> purification by gel filtration, ion exchange, and/or affinity
> chromatography.  Preferably, the protein should have an activity that can
> be assayed easily and cheaply.  The starting material should be something
> that's clearly recognizable as "life".  Unfortunately, the number of steps
> has to be very limited, as time does not allow anything particularly
> complicated.  Also, it has to be doable at room temperature.
> Any ideas, good references, or protocols?

>Ron Tate                                                                   
>Lab of Franklin Leach
>Dept. of Biochem. & Molecular Biology
>Oklahoma State University rtate at bmb-fs1.biochem.okstate.edu
(405) 744-9326

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