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Germ line expression

Ananth Badrinath asbadrin at students.wisc.edu
Tue Mar 5 21:02:47 EST 1996

   I am interested in looking at the dynamics of the Endoplasmic reticulum
following fertilization in C. elegans embryos.  In order to visualize it I
need a very probe specific for this organelle-I have a constrct with GFP,
followed by the ER retention sequence "KDEL"  My current strategy has been
to make mRNA in vitro and inject it.  
   I am wondering if it is possible to get germline expression of GFP by
injecting the plasmid.  From what I gather most such injected DNA
typically ends up in a extrachromosomal array that can be stably
inherited; however, germline expression is not seen.  If I go for a direct
integration (even though it is very rare) would I be able to get germline
exprssion?  What kind of a promoter would I need to use?  
   Would appreciate any feedback.  Thanks in advance.

Yours sincerely,

Ananth Badrinath

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