Hi there,
got some problems with blast searches. Screened for some C. elegans ESTs
and did a Blast search. Got hits mainly with cosmids. However, some
clones are on the minus strand of the cosmids and GENEFINDER did not see
them, although they are obvious genes. All the Genes in the plus strand
were identified.
My Question: Does GENEFINDER search only plus strands? How do I identify
the other clones? Please help. I'm desperate. I got mainly 3' non-coding
regions of the ESTs (300 - 500 bp).
Thanx
