>From The Worm Breeder's Gazette, 1998, v. 15, No. 2, p. 56.
AN ATTEMPT TO SLOW AGING IN C. elegans.
7. NO EFFECT OF SODIUM ADENOSINETRIPHOSPHATE
Vladimir V. Bakaev, box 45, Novosibirsk, 630107, USSR
The purpose of this study was to investigate the effect of
different concentrations of sodium adenosinetriphosphate in
water solutions on nematode life span. In this experiment
sodium adenosinetriphosphate was used in following diluti-
ons: 1:10(3), 1:10(4), 1:10(5), 1:10(6) and 1:10(7). Three
adult animals (3 - 5 days old) were kept in microtitre wells
containing 0,75 ml of liquid medium (with E. coli and witho-
ut sodium adenosinetriphosphate) during 4 hours, then they
were discarded and newborn larvae were transferred in next
wells (with sodium adenosinetriphosphate in any concentrati-
on) every day (one worm in one well). This investigation was
carried out in temperature +21oC and in the darkness. The
obtained results are presented in the following table.
=C7=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=E0=
=C4=C4=C4=C4=C4=E0=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=
=C4=C4=C4=C4=C4=C4=C4=C4=C4=C9
=C5 Concentration of =C5 =C5 Longevity (days) =C5
=C5 sodium =C5 =DC=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=
=C4=E0=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=E1
=C5 adenosinetriphosphate =C5 n =C5 mean+/-S.E. =C5 maximal =C5
=DC=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=E4=
=C4=C4=C4=C4=C4=E4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=E4=C4=C4=C4=C4=
=C4=C4=C4=C4=C4=C4=C4=C4=C4=E1
=C5 Control =C5 12 =C5 20,8+/-1,7 =C5 27 =C5
=C5 =C5 =C5 =C5 =C5
=C5 1:10(3) =C5 12 =C5 5,6+/-0,2 =C5 6 =C5
=C5 =C5 =C5 =C5 =C5
=C5 1:10(4) =C5 12 =C5 6,1+/-0,2 =C5 8 =C5
=C5 =C5 =C5 =C5 =C5
=C5 1:10(5) =C5 12 =C5 10,7+/-1,2 =C5 20 =C5
=C5 =C5 =C5 =C5 =C5
=C5 1:10(6) =C5 12 =C5 20,7+/-1,3 =C5 27 =C5
=C5 =C5 =C5 =C5 =C5
=C5 1:10(7) =C5 12 =C5 20,9+/-1.8 =C5 26 =C5
=D1=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=E2=
=C4=C4=C4=C4=C4=E2=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=E2=C4=C4=C4=C4=
=C4=C4=C4=C4=C4=C4=C4=C4=C4=D6
The nematodes in concentration of sodium adenosinetriphosp-
hate 1:10(3) were unable to have progeny, then, the concent-
rations 1:10(3) and 1:10(4) were toxic. The concentration
1:10(5) was slightly toxic (the differences between longevi-
ties in concentrations 1:10(3), 1:10(4), 1:10(5) and in
control were statistically significant, P < 0,001 in all
these cases). The longevities in concentrations 1:10(6) and
1:10(7) and in control were similar (the differences between
longevities in concentrations 1:10(6) and 1:10(7) and in
control were statistically not significant, P > 0,05 in all
these cases).
Conclusion: If the water solution of sodium adenosinetrip-
hosphate was applied to C. elegans during the whole life
span in above described conditions, it was not able to pro-
long the life span of these animals.
Acknowledgment: The author wishes to express his thanks to
CGC for providing C. elegans (Bristol, N2) and E. coli OP50.
