Hello,
I previously posted a query for results of integration screens that use UV
or oligo-mediated integration. I received many replies, some of which
suggested using the mutagen MMS as an alternative. I have posted some of
these, and a short compendium of other (hopefully useful) information, at
the following URL:
http://deficiency.lscf.ucsb.edu/int.html
Thanks to all who replied. If you would like to suggest corrections or
additions to this web page, please let me know.
[As an aside, I was able to integrate 3/3 lines (0.7% frequency) using a
gamma cell at UCLA (thanks to Arnaud Labrousse for the help!).]
Regards,
Morris
Morris Maduro
Department of MCD Biology
Biological Sciences II
UC Santa Barbara
Santa Barbara, CA 93106
lab: (805) 893-8090
fax: (805) 893-2005