Hi chris. My name is gidi shemer and I'm also after my first year in the
phd (I'm working in the technion, israel in beni podbilewicz lab). I
have'nt tryed the soaking method but if you want some details, I thing
you should write to Ron Eliss. In the last meeting he showed some nice
staff about this method with the gene fem-1. As for the positive control,
you can use apx-1. You should get dead embryos arested at about 50 cells
stage with hyper pharyngal cells. If you want more details, e.mail me at
work (bishemer at tx.technion.ac.il). bye, gidi.
> As a first year PhD student who is currently working with dsRNA
> interference in C.elegans i was interested to see in some of the
> abstracts at the 1999 International Worm Meeting that a number of
> people were using dsRNA soaking as a method of RNAi instead of
> I dont seem to be able to find anything further on the technique so i
> was hopeing that someone may be able to fill me in on how its done
> and how successful it is compared to microinjection of dsRNA.
> I would also like to know if anybody has a good gene to use as a
> positive control in dsRNA injections as the genes i am looking at
> have not been used before and so i have no idea if they give an
> obvious phenotype. It may be that my injection technique needs work
> so a positive control with a good phenotype would help.
>> Any help would be great
> Chris Mee
> University of Nottingham
>plxcjm at nottingham.ac.uk> ---