The chemotaxis assay as described by Sam Ward works very well, but there
are other assays that people have worked out described in:
Bargmann CI, Hartwieg E, Horvitz HR. Odorant-selective genes and neurons
mediate olfaction in C. elegans. Cell. 1993 Aug 13;74(3):515-27.
Bargmann CI, Horvitz HR Chemosensory neurons with overlapping functions
direct chemotaxis to multiple chemicals in C. elegans. Neuron 1991
Nov;7(5):729-42
Pierce-Shimomura JT, Morse TM, Lockery The fundamental role of
pirouettes in Caenorhabditis elegans chemotaxis. J Neurosci. 1999 Nov
1;19(21):9557-69.
Wicks SR & Plasterk RHA High-Gradient Soluble-Compound Chemotaxis Assay.
on the web page:
http://www.nki.nl/nkidep/h8/protocols/gradtax.html
Best of luck! - jon
On Wed, 16 Aug 2000 destasie at lawrence.edu wrote:
> We are wondering if there are more recent protocols for chemotaxis
> assays
> than those published by Ward.
>> De Stasio lab
>Elizabeth.A.Destasio at lawrence.edu>>> ---
>>>>
Jon Pierce-Shimomura
Institute of Neuroscience jon at chinook.uoregon.edu
1254 University of Oregon phone: (541) 346-4592
Eugene, OR 97405 fax: (541) 346-4548
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