I am a postdoc. working in non-wrom background Lab. Recently I identyfied
and characterized a phophalipase homologe in C.elegans and it might also
exist in germline because it was first found in testis.
I meet a problem in doing in situ hybridization with whole mount C.elegans.
most time I do the in situ , the result is not very consistant.
To publish this paper soon, I am looking for some people who is skill at in
situ hybridization techneque to cooperate with.
Any one who would like to help will be highly apprecitaed and be a auther of
this article.
Please reply to:
zhaobw at u.washington.edu
Juan <abrah023 at gold.tc.umn.edu> wrote in message
news:8ecuiu$ff7$1 at mercury.hgmp.mrc.ac.uk...
> Hi, I am wondering if someone has performed RT-PCR on sigle worms using a
> light cycler. If so, could you please provide with some tips/protocol. I
> would eternally thank you for it.
>> Juan
> "Rougvie Lab"
> ---
---
