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S basal solution, and starvation treatment

Kleemann kleemann at cricket.unl.edu
Thu Nov 23 12:32:38 EST 2000

Dear C. elegans community:

    I would like to subject my worms to a starvation treatment. From the
references that I have read, a NG solution has been used in the past for
this sort of treatment.  I have found recipes for a S basal and S
complete solution.  I was wondering if the S basal solution can be used
to support the worms for a short period (6hrs) during the starvation
(just S basal)  and control treatments (S basal plus ~.5 g of live e.
coli) for my experiment. If it is inadvisable to use just S basal alone,
do you know of any commercial distributor of S complete or any of its
constituents in a premixed form.

Thank you for any suggestions
Happy thanksgiving

Gunnar Kleemann
kleemann at cricket.unl.edu
University of Nebraska - Lincoln


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