From Miriam Goodman and Shawn Lockery
We regret to inform the C. elegans community that the published recipe for
internal saline for whole-cell recordings[1,2] from neurons contains an
error (see below for both the published and correct recipes).
published correct
KGluc 125 mM 125 mM
KCl 18 18
NaCl 4
CaCl2 0.7 0.6
MgCl2 1 2
HEPES 10 10
EGTA 10 10
The main effect of this error resides in the difference in NaCl
concentration. The correct saline will produce a predicted ENa = 000 mV
with the published external saline, while the erroneous published saline
has an undefined ENa. Because C. elegans lacks voltage-gated Na channels,
this difference in salines may have little or no effect on recordings of
voltage-gated currents. It may, however, affect measurements of currents
carried by ligand-gated currents and currents carried by DEG/ENaC
channels. We apologize for any inconvience this error may have caused.
1. Goodman, M.B., Hall, D.H., Avery, L., and Lockery, S.R. (1998) Active
Currents Regulate Sensitivity and Dynamic Range in C. elegans Neurons.
Neuron 20:763-772.
2. Lockery, S.R. and Goodman, M.B. (1998) Tight-seal whole-cell patch
clamping of C. elegans neurons. Methods in Enzymology 295:201-217.
---
