I am trying to figure out what cells my gene of interest is
expressed in, using a GFP fusion. In order to help figure out what
cells I'm seeing expression in, I want to do a double-labeling
experiment with my GFP fusion and an antibody stain (to LIN-26) as a
marker. I'm assuming GFP is killed by fixation; am I right? And if
so, does anyone have advice about what commercially available
anti-GFP antibodies work well to stain embryos?
Thanks for your help!
Dept. Biology and Biotechnology
Worcester Polytechnic Institute
100 Institute Rd.
Worcester, MA 01609