Dear Worm Breeders,
I have been faced with a problem regarding injection pads. I have been
unable to make pads that are sticky. As a consequence, the worms soon begin
to move about, and this makes the injections difficult. I have experimented
with different brands and concentrations of agar, but nothing makes a
significant difference. I follow the injection protocols described in the
"red c. elegans methods" book. Other than this problem with non-stick pads,
the injections go fairly well- the needles are sharp with a nice flow rate,
and the worms typically survive and generate rollers efficiently. If anyone
has any comments or advice, I would greatly appreciate hearing it.
Thank you,
Craig Mackinnon
UIUC
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