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How do I get many eggs?

Nobody nobody at hgmp.mrc.ac.uk
Sat Nov 24 10:32:00 EST 2001

I am trying to grow large numbers of gravid adults in liquid culture, to
isolate eggs. Apart from growing  a synchronized culture (starting with
eggs/L1) does anyone know of a trick to increase the proportion of gravid
adults in liquid cultures?

Is it possible, for example, to start cultures with less food (I usually use
500 mL worth of NA22 per 500 mL of S-basal), so that they starve & form
dauer, and then to start them up again, so they all hit adulthood but the
same time?

Any help would be greatly appreciated

Dan Jeffares

Dan Jeffares

Department of Evolutionary Biology
Zoological Institute
University of Copenhagen
Universitetsparken 15
DK-2100 Copenhagen =D8
Phone +45 35321278 (W)/38193924 (H)
=46ax +45 35321300
Email: dcjeffares at zi.ku.dk


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