In our lab we freeze C.elegans in liquid nitrogen using the method
described in: The nematode Caenorhabditis elegans, William B. Wood, Cold
Spring
Harbor Laboratory, 1988.
If you have problems getting a copy, poste again and I will translate my
german copy for you.
Greetings, Vera Lahl
"Sean Jabbar" schrieb in Nachricht ...
>Hi,
>>Is there anyone who can provide a detailed, working protocol for freezing
C.
>elegans in liquid nitrogen? We currently are freezing them at -70 C, but
for
>some reason they do not survive when placed in the liquid nitrogen using
our
>current protocol.
>>Thanks in advance,
>>Sean Jabbar
>>s-jabbar at northwestern.edu>>---
>
