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embryo lysis

Igor Antoshechkin igor.antoshechkin at colorado.edu
Wed Jan 9 17:45:54 EST 2002


I am trying to do a Western blot on a small number of embryos. Because
of the small sample volume, I cannot use a sonicator, and just boiling
in SDS loading buffer does not seem to lyse embryos well. If somebody
has a better way to do that, I'd appreciate if you let me know.

Thanks a lot.
Igor.

Han Lab
University of Colorado at Boulder.

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