To all,
I have summarized in this message the information I have
received or gathered
regarding Golgi markers in C. elegans. Details are found in the papers I
have referenced and/or by corresponding with the authors:
1) GFP fusions to Golgi enzymes have been made and when expressed in some
tissues stain punctate structures in the cytoplasm. See figure 1 of:
Rolls et al. (2002). Targeting of rough endoplasmic reticulum
membrane proteins
and ribosomes in invertebrate neurons. Mol. Biol. Cell 13, 1778-1791.
2) From: Patricia Berninsone <pbernins at bu.edu>
Subject: Golgi markers
I am not aware of any that expresses in all worm tissues. But depending on
what you need, you may want to look into the sqv (squashed vulva) genes.
Some of them encode for proteins involved in glycosaminoglycan
biosynthesis, which is known in mammalian cells to occur in the Golgi. An
epitope tagged version of SQV-7 -a nucleotide sugar transporter- clearly
localized to the Golgi when I expressed it in MDCK cells. Of course this
may only mean that the protein has some Golgi localization signal
recognizable across species. Ho-Yon Hwang from the Horvitz lab reported
"punctate cytoplasmic" staining with polyclonal antibodies against SQV-7
(hypodermal seam and gonadal distal tip cells), and SQV-5 (vulva and
germline) at the 2001 International Worm Meeting abstract 188. You may want
to check with him.
3) BODIPY-BFA (Brefeldin A) labels all cytoplasmic membrane compartments
and BODIPY-ceramide is supposed to label Golgi specifically. These have
been used to assay these compartments during cytokinesis in live embryos
(see Figure 3 in reference below). I do not know if these will work on fixed
specimen. The chemicals were purchased from Molecular Probes and
their Technical
Department might know. Reference:
Skop et al. (2001). Completion of cytokinesis in C. elegans requires
a brefeldin
A-sensitive membrane accumulation at the cleavage furrow apex. Current Biology
11, 735-746.
4) LIN-10 has been reported to strongly label at or near the Golgi and more
weakly in the cytoplasm and at the plasma membrane. However, LIN-10
is specifically
expressed in vulval epithelial cells and in neurons. Reference:
Whitfield et al. (1999). Basolateral localization of the Caenorhabditis
elegans epidermal growth factor receptor in epithelial cells by the PDZ protein
LIN-10. Mol. Biol. Cell 10, 2087-2100.
Thank you very much for all who sent me input. If any new developments
happen, please let the community know about it.
Hanna (Johnny) Fares
University of Arizona
Department of Molecular and Cellular Biology
Life Sciences South Building, Room 531
1007 East Lowell Street
Tucson, AZ, 85721
Tel (Office): 520 626 3759
Tel (Lab): 520 626 5996
Fax: 520 621 3709
e-mail: fares at email.arizona.edu
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