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cDNA isolation/amplification

Hanna Fares fares at email.arizona.edu
Mon Jul 14 12:53:46 EST 2003

	I have succeeded in isolating a pitifully small number 
(~100-500) of active coelomocytes from worms.  I would like to 
isolate mRNA and make cDNA from these.  Does anybody have or know of 
good protocols to isolate/amplify cDNA from a small number of cells?

Hanna (Johnny) Fares
1007 East Lowell Street
Department of Molecular and Cellular Biology
University of Arizona
Tucson, AZ, 85721
TEL (office) :  520 626 3759
TEL (lab) :  520 626 5996
FAX:  520 621 3709


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