I have succeeded in isolating a pitifully small number
(~100-500) of active coelomocytes from worms. I would like to
isolate mRNA and make cDNA from these. Does anybody have or know of
good protocols to isolate/amplify cDNA from a small number of cells?
Hanna (Johnny) Fares
1007 East Lowell Street
Department of Molecular and Cellular Biology
University of Arizona
Tucson, AZ, 85721
TEL (office) : 520 626 3759
TEL (lab) : 520 626 5996
FAX: 520 621 3709
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