We routinely isolate deletion mutants using TMP/UV. Our procedure is
"lab-base", and may not be suitable for genome-wide project. Once we decide
to knock out a particular gene, we are able to recover a deletion mutant in
two weeks by screening our frozen library.
If someone is interested, please go to the following address in our home page.
Laboratory of Molecular Neurobiology
Division of Biological Science ???
Graduate School of Science, Nagoya University
Nagoya 464-8602, Japan????
email: m46920a at nucc.cc.nagoya-u.ac.jp?????