Dear All:
What is the current consensus on how to determine the expression
pattern of
genes that reside in an operon in the second, third, etc position? One
way to
approach this problem that seems to be reasonable is to take a genomic
sequence
from the end of the nearest to the operon 5' gene to the beginning of
the
nearest 3' gene and then replace the gene of interest with a GFP
sequence. Is
this a viable strategy?
I am going through the literature to see what has been done, but it
would be
nice to receive a bolus of concentrated knowledge on this subject.
Thank you very much for your help
Nicholas Lyssenko
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American Heart Association Predoctoral Fellow
The Huck Institute for Life Sciences and
Department of Biochemistry and Molecular Biology
513 Wartik Lab
Penn State University
University Park, PA 16802
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