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[Celegans] Re: Phage contamination of HT-115 RNAi bacteria?

Satoru Uzawa satoru at meyer11.berkeley.edu
Thu Oct 20 23:28:59 EST 2005

   Just for a case no one responded to your query...

   Since the phage is infecting HT-115 but not OP50 and it came from 
nowhere, I suspect it may be the notorious T1 phage (very resistant to 
drying up).  Some lab strains are T1-resistant but some are not.  
Since getting rid of T1 phase is not that easy, my recommendation is to 
use other strains that are pronounced to be T1-resistant.  In old days, 
people sprayed the whole lab with cresol or phenol to get rid of T1 
contamination! (At least I heard so.)  

   Good luck!

   Satoru Uzawa
   Meyer lab, UC Berkeley 

Laura Mitic <lmit7870 at itsa.ucsf.edu> wrote:
> Hi,
> We have recently experienced an outbreak of phage in lawns of HT-115 
> RNAi-expressing bacteria seeded on NG-carb plates.  The phage 
> contamination is at very low levels (1-2 plaques per lawn, on about 1 
> lawn out of 5, affecting only about 5 of 20 lab personnel) and seems 
> to be specific to HT-115 bacteria since OP50 is not affected.  The 
> plaques are clean-edged and uniform in shape. Thus far we have been 
> completely unable to trace the source.  I'm wondering if anyone else 
> has experienced phage contamination, where it came from, what you've 
> done to prevent it from reoccurring?
> Thanks in advance,
> Laura Mitic
> Kenyon Lab, UCSF
> lmit7870 at itsa.ucsf.edu

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