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[Celegans] Removal of external bacteria from C. elegans

Sarah Perkins sep18 at psu.edu
Mon Jun 12 08:41:25 EST 2006

Dear All
I am looking for a method for removing bacteria that may have adhered
externally to C. Elegans, but will not remove ingested bacteria that may
have established an infection in the worm. 
I have tried washing worms with antibiotics for differing lengths of time,
but the worms appear to ingest this and the internal bacteria are also
destroyed. I also tried washing them in chlorinated tap water, and
separately, a rinse in ph 4. The last was maybe the most effective, but
still doesn't remove all external bacteria. I plan to test the efficacy of
external removal of bacteria using confocal microcopy. If anyone has any
established methods for external removal of bacteria I would be very
interested to hear from them. Any further ideas will be much appreciated. 
Best, Sarah 

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