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[Celegans] Real Time PCR

g.Joshua from lshtm.ac.uk via celegans%40net.bio.net (by g.Joshua from lshtm.ac.uk)
Thu Jan 8 07:11:07 EST 2009

Dear colleagues,
Is there a consensus on best/good housekeeping gene in C. elegans to use 
to normalise against in Real Time PCR?
Have tried act-3 (see Park et al (2007) J Mol Biol 372 (2), 331-340) but 
find large differences in RNAs from two worm populations (in all replicates).
Best regards,
G. Joshua.
London School of Hygiene and Tropical Medicine
Dept. Infectious and Tropical Diseases,
Keppel St.,
London WC1E 7HT.
Tel:     (+44) (0)20 7927 2028
FAX:    (+44) (0)20 7637 4314
E-mail:  g.joshua from lshtm.ac.uk

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