Is there a consensus on best/good housekeeping gene in C. elegans to use
to normalise against in Real Time PCR?
Have tried act-3 (see Park et al (2007) J Mol Biol 372 (2), 331-340) but
find large differences in RNAs from two worm populations (in all replicates).
London School of Hygiene and Tropical Medicine
Dept. Infectious and Tropical Diseases,
London WC1E 7HT.
Tel: (+44) (0)20 7927 2028
FAX: (+44) (0)20 7637 4314
E-mail: g.joshua from lshtm.ac.uk