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[Celegans] help with DAPI fluorescence project please

Andrew Papp via celegans%40net.bio.net (by ap from tritechresearch.com)
Wed Jan 14 18:44:48 EST 2015


Hi,

I am developing a low-cost LED-based fluorescence dissecting scope, 
specifically for worm work. I've already got excellent results with eGFP 
and mCherry that I will present at the next worm meeting, but would like 
to extend the system to show DAPI staining as well. I have live strains 
that express the proteins, but in my small lab, I don't have any DAPI or 
the items to conveniently mount worms and DAPI stain them or even any 
DAPI to stain worms.

I would really appreciate it if someone could send me some old slides 
(or make me a new slide) with some DAPI stained worms. They can be WT, 
mutant, any stage... even embryos, as long as they show bright DAPI 
staining of nuclei.  I can pay the shipping if necessary and can return 
the slides within a few weeks if desired.  I will promise not to show 
the images of the slides anywhere, if that is important. Alternatively, 
a little DAPI in a tube and a simple protocol or link to one would be 
okay, too.

Any help would be greatly appreciated.

Thank you!!!!

Andy

-- 
Andrew Papp, Ph.D.
Tritech Research
2961 Veteran Avenue
Los Angeles, CA   90064
Tel: (310)441-3767
ap from TritechResearch.com




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