IUBio GIL .. BIOSCI/Bionet News .. Biosequences .. Software .. FTP

[Celegans] help with DAPI fluorescence project please

Andrew Papp via celegans%40net.bio.net (by ap from tritechresearch.com)
Wed Jan 14 18:44:48 EST 2015


I am developing a low-cost LED-based fluorescence dissecting scope, 
specifically for worm work. I've already got excellent results with eGFP 
and mCherry that I will present at the next worm meeting, but would like 
to extend the system to show DAPI staining as well. I have live strains 
that express the proteins, but in my small lab, I don't have any DAPI or 
the items to conveniently mount worms and DAPI stain them or even any 
DAPI to stain worms.

I would really appreciate it if someone could send me some old slides 
(or make me a new slide) with some DAPI stained worms. They can be WT, 
mutant, any stage... even embryos, as long as they show bright DAPI 
staining of nuclei.  I can pay the shipping if necessary and can return 
the slides within a few weeks if desired.  I will promise not to show 
the images of the slides anywhere, if that is important. Alternatively, 
a little DAPI in a tube and a simple protocol or link to one would be 
okay, too.

Any help would be greatly appreciated.

Thank you!!!!


Andrew Papp, Ph.D.
Tritech Research
2961 Veteran Avenue
Los Angeles, CA   90064
Tel: (310)441-3767
ap from TritechResearch.com

More information about the Celegans mailing list

Send comments to us at archive@iubioarchive.bio.net