On 13 Apr 1995, Frederic Bourgaud wrote:
> Hi all,
>> I am starting a new research program dealing with morphine in plants. I am
> looking for a rapid/reliable ELISA test that would be adapted to this. I
> already know that ELISA tech.(Lexington, Kentucky) commercialises a kit
> called "generic opiate ELISA test". It's a competition test and involves
> polyclonal antobodies. It is not very specific to morphine as it crosses
> with all the related alkaloids. It costs about 90 US $ per plate.
>> Does anybody know another ELISA test, maybe based on monoclonal antibodies
> ? It is important to me to have a test more specific to morphine. Also any
> experience/reference on morphine quantification in plants with ELISA would
> be appreciated.
>> Also posted to the Biotech and Plant-TC discussion lists. Sorry for
> multiple postings.
>> Thanks
>> Fred (bourgaud at ensaia.u-nancy.fr)
For tests for morphine and other drugs of abuse, you can try the following:
Hanson Hog Biomedical Co., Taipei, Taiwan, Tel +886-2-7922808, Fax
..7914624
Immunotech Corp, Boston, MA, Tel +1-617-787-1010, Fax ..787-0315
Princeton Biomeditech, Princeton, NJ, Tel +1-908-274-1000, Fax ..274-1010
BiosPacific, Emeryville, CA, Tel +1-415-652-6155, Fax ..652-4531
Eucardio Laboratory, San Diego, CA, Tel +1-619-689-8719, Fax ..689-8719
(The above addresses are from my own database, so some or all may be
outdated).
As to the specificity of the assay, I think that in your case, less
specificity is preferable for initial screening. Once you find a plant
with traces of your material, try to determine the exact molecule.
Falk Fish, Tel-Aviv, Israel.
