I am planning to use mouse thymocytes as feeder cells for hybridomas.
The thymus donors are now a month old, but the fusion... well I haven't
even started immunizing yet. I was wondering whether it is possible to
freeze feeder cells and thaw them a day or so before the fusion. I'd
really rather use thymocytes than spleenocytes, so I have to decide
on this within the next week or so. Please let me know if you've tried
this, how it worked, protocols, etc.
Thanks,
Chris <Rampitsch at bcrssu.agr.ca>
