On 21 Apr 1995, Robert Burns wrote:
>> Hi all,
> have any of you had a go at generating antibodies using
> lambda phage expression vectors. I know that one main advantage
> is the numbers of antibodies generated but what are the disadvantages?
> Thirdly does anyone know of an easy-to-use kit which can be used for
> this technology?
>> Thanks
>> Robert
>>>>>> Robert Burns
>The main problem of this method is the low affinity of the resulting
antibodies.
I did not have direct experience with the method but we keep it in the
back of our minds as a viable strategy to generate antibodies, while
combining it with an approach to ensure that the affinity will be usably
high.
As to a kit: I think Pharmacia offers a kit. I am not sure and I will
get back after looking at my papers.
You could also contact the guys at Cambridge: they have invented it all.
Falk Fish, Tel-Aviv, Israel
