We routinely make monoclonal AP conjugates by thiolation of the
antibody followed by reaction with activated AP. We find that thge
storage conditions for each conjugate is dependent on the Mab used.
Some conjugates store well at 4C, some don't. All of them store well
at -20C. We have also been looking at a Pierce product called
superfreeze which allows liquid storage of conjugates at -20C which
should increase longevity. We find that some conjugates store better
with it and some without. BTW in some cases conjugates stored with
superfreeze had decreased activity compared to aliquots of the same
batch stored at 4C without it!
I think with Mab conjugates it all depends on the parent antibody.
Anybody else got any conjugate stories?
Monoclonal Antibody Unit
Scottish Agricultural Science Agency
burns at sasa.gov.uk