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Single substrate component for TMB

Randy Leavitt rleavitt at on.novamann.ca
Tue Aug 1 16:10:25 EST 1995

In article <199507161426.WAA27105 at einstein.technet.sg>, pohyam at TECHNET.SG 
>I have always been using the two component OPD and BCIP/NBT coupled with 
>for my ELISA assays. Recently, I read that a new single component TMB 
>substrate for HRP is on sale. Has anyone tried this? Is it superior in 
>of OD duplicity and stability of colour development? Can someone share 
>experience with me?

We have used the single component TMB substrate for the past 2 years in 
our single step ELISAs for drugs in the race horse.  Use of the product 
(K-Blue) has improved sensitivities, precision and made for easier 
handling than the classical 2 component system.

K-Blue is available from ELISA TECHNOLOGIES in Lexington, KY 

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