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Problems with DNA based technology

Michael Douglas douglasm at SIAL.COM
Sun Aug 6 15:25:40 EST 1995

It is essential with these sensitive methodologies that everything 
including glassware, plasticware and reagents be RNAse free.  Treat all 
reagents and solutions with DEPC (diethylpyrocarbonate), wear gloves use 
good laboratory practice as if sterile and pray.  MGD

Michael Douglas					   	  douglasm at sial.com
Sigma Diagnostics    --    545 South Ewing    --    St. Louis, MO 63103 USA
FAX: 314-531-2586 or 800-816-1603  Tele: 800-521-8956 or 314-771-5765 x4435

On 5 Aug 1995, Nadagen wrote:

> My laboratory has had a slight problem with the logistics of running PCR
> because of the ribonuclease destroying the m-RNA before the appropriate
> info can be gathered....do you know how to stabilize this or have any
> suggestions

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