It is essential with these sensitive methodologies that everything
including glassware, plasticware and reagents be RNAse free. Treat all
reagents and solutions with DEPC (diethylpyrocarbonate), wear gloves use
good laboratory practice as if sterile and pray. MGD
___________________________________________________________________________
Michael Douglas douglasm at sial.com
Sigma Diagnostics -- 545 South Ewing -- St. Louis, MO 63103 USA
FAX: 314-531-2586 or 800-816-1603 Tele: 800-521-8956 or 314-771-5765 x4435
On 5 Aug 1995, Nadagen wrote:
> My laboratory has had a slight problem with the logistics of running PCR
> because of the ribonuclease destroying the m-RNA before the appropriate
> info can be gathered....do you know how to stabilize this or have any
> suggestions
>>
