gwade at physiology.uwo.ca <gwade at physiology.uwo.ca> wrote:
>>> I just found a great new product from Kalyx Biosciences. AMPLISCRIPT
>>> allows you to detect your PCR products using an ELISA format.
>No labelled primers.
>Works on 96 welled plates.
>So now you have many copies of RNA which is complementary to the DNA
>you were probing for.
>>Kalyx has some literature outlining this process.
>>Interestingly enough they also have an inexpensive matrix on which you
>can do a nucleic acid hybridization in about 90 min (at room temp).
>Hi,
The process seems interesting, but can it be used for the detection of small
PCR fragments, 100 to 300 pb, used to detect mRNA ?
And can it be used to quantified finally the mRNA ?
Luc
Luc Ferrari
Centre du Medicament - URA CNRS 597
Faculte de Pharmacie - Universite Henri Poincare
Nancy, France
