Hello Mario,
The restriction nuclease you are after with recognition sequence:
RG/CY is CviJI, which is produced by a large DNA virus of a
eukaryotic alga (Chlorella sp.). This and related viruses encode a
number of restriction and modification systems, some with unique
recognition sequences. These enzymes were originally discovered here
at UNL in Jim Van Etten's lab. They are commercially available from
the following company:
Megabase Research Products
2820 N. 48th Street, Suite 110
Lincoln, NE
Phone: (402) 467-6499
Fax: (402) 472-2853
As you point out, frequent cutters like CviJI are ideal for analysis
of short PCR products--I've used it to distinguish strains of a plant
virus.
Best Wishes,
Roy French, USDA, ARS
