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roy french rfrench at UNLINFO.UNL.EDU
Wed Dec 13 11:25:40 EST 1995

Hello Mario,

The restriction nuclease you are after with recognition sequence: 
RG/CY is CviJI, which is produced by a large DNA virus of a 
eukaryotic alga (Chlorella sp.). This and related viruses encode a 
number of restriction and modification systems, some with unique 
recognition sequences. These enzymes were originally discovered here 
at UNL in Jim Van Etten's lab. They are commercially available from 
the following company:

Megabase Research Products
2820 N. 48th Street, Suite 110
Lincoln, NE

Phone: (402) 467-6499
Fax:   (402) 472-2853

As you point out, frequent cutters like CviJI are ideal for analysis 
of short PCR products--I've used it to distinguish strains of a plant 

Best Wishes,
Roy French, USDA, ARS

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