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Automated/simple DNA extraction

John Krolewski jjk5 at COLUMBIA.EDU
Wed Dec 13 10:40:12 EST 1995


I am looking for ways to speed extraction of DNA for use in Southern
blotting and PCR based diagnostic tests.

1. Can anybody provide info/critique on automated DNA extraction equipment
(high molecular weight)?  How do these compare, w.r.t. time, yield and DNA
quality, against reagents like DNAzol?

2. Can anybody provide info/critique on matrices such as S&S IsoCode for
simple isolation of DNA from blood (low molecular weight suitable for PCR).






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