PCR and LCR are based on thermostable DNA polymerase and ligase
respectively. In the dog days between Christmas and New Year I find
myself wondering what other sequence-specific amplification methods
could be devised if suitable enzymes were available. Can anyone tell me
if there is, for example, a thermostable RNA polymerase commercially
available? It seems to me that this would greatly simplify
3SR/NASBA.
Input/ other ideas welcomed.
Kevin
Dr Kevin O'Donnell "Work as if you were in the early days
Diagnostics and Molecular Biology of a better nation"
SASA - Alasdair Gray
Edinburgh
