You are absolutely right as to the utility of thermostable enzymes for
working with RNA; and that there currently aren't any available. We (Life
Technologies/ GIBCO BRL) are trying to engineer a thermostable reverse
transcriptase. So far, we're still trying. If you'd like, I'd be glad
to put you in contact with the project leader.
Have a great 1996.
Rich Schifreen phone: 301-840-4163
Life Technologies fax: 301-670-1493
E-Mail: rschifre at access.digex.net
On 28 Dec 1995, Dr Kevin O'Donnell wrote:
> PCR and LCR are based on thermostable DNA polymerase and ligase
> respectively. In the dog days between Christmas and New Year I find
> myself wondering what other sequence-specific amplification methods
> could be devised if suitable enzymes were available. Can anyone tell me
> if there is, for example, a thermostable RNA polymerase commercially
> available? It seems to me that this would greatly simplify
> 3SR/NASBA.
>> Input/ other ideas welcomed.
>> Kevin
>>> Dr Kevin O'Donnell "Work as if you were in the early days
> Diagnostics and Molecular Biology of a better nation"
> SASA - Alasdair Gray
> Edinburgh
>>
