Brian,
We have recently completed a comparison study of malignant histiocytosis
and anaplastic giant cell carcinoma in the canine. For the
immunohistochemical studies, we used both anti-alpha 1 antitrypsin and
anti-lysozyme. Both of these markers worked well, but in general, the
signal from the anti alpha 1 antitrypsin was the strongest. The paper
can be found in the journal: Veterinary Clinical Pathology 23:118-122,
1994. If you have any further questions on the technique, please call or
e-mail.
Good luck!
*********************************************************************
* Dave Getzy
* Director
* CSU Diagnostic Laboratories
* 300 West Drake, Fort Collins, Co 80523
* PHONE 303-491-1281 FAX 303-491-0320
* EMAIL dgetzy at vth1.vth.colostate.edu
********************************************************************
On 15 Mar 1995 CHELACK at admin3.usask.ca wrote:
> Hi, I keep getting requests for a staining paraffin sections for macrophages
> in particular cases of suspected histiocytic tumors in dogs. What we are
> looking for is a marker for macrophages/histiocytes in paraffin sections
> which could be used in any species. Is there anyone out there in netland
> who is already doing this? My initial thoughts are that an antibody to
> myeloperoxidase or alpha 1 anti tryosin might be the way to go but I am
> short on time and wonder if anyone has validated such an antibody on paraffin
> sections, or for that matter in other species. Most of the commercially
> available antibodies such as Mac-1 or Mac-3 do not seem to work on paraffin
> sections of canine tissues so I am looking for a more generic marker.
> Thanks for any advise.
>> Regards
> BJC - Brian Short people... last to know its
> raining, first to know its a flood.
>>
