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Immunohisto marker for macrophages needed

David Getzy dgetzy at VTH1.VTH.COLOSTATE.EDU
Mon Mar 20 10:28:21 EST 1995


We have recently completed a comparison study of malignant histiocytosis 
and anaplastic giant cell carcinoma in the canine.  For the 
immunohistochemical studies, we used both anti-alpha 1 antitrypsin and 
anti-lysozyme.  Both of these markers worked well, but in general, the 
signal from the anti alpha 1 antitrypsin was the strongest.  The paper 
can be found in the journal:  Veterinary Clinical Pathology 23:118-122, 
1994.  If you have any further questions on the technique, please call or 

Good luck!

* Dave Getzy
* Director
* CSU Diagnostic Laboratories 
* 300 West Drake, Fort Collins, Co  80523 
* PHONE 303-491-1281  FAX 303-491-0320
* EMAIL dgetzy at vth1.vth.colostate.edu 

On 15 Mar 1995 CHELACK at admin3.usask.ca wrote:

> Hi, I keep getting requests for a staining paraffin sections for macrophages
> in particular cases of suspected histiocytic tumors in dogs.  What we are 
> looking for is a marker for macrophages/histiocytes in paraffin sections 
> which could be used in any species.  Is there anyone out there in netland
> who is already doing this?  My initial thoughts are that an antibody to 
> myeloperoxidase or alpha 1 anti tryosin might be the way to go but I am 
> short on time and wonder if anyone has validated such an antibody on paraffin
> sections, or for that matter in other species.  Most of the commercially
> available antibodies such as Mac-1 or Mac-3 do not seem to work on paraffin
> sections of canine tissues so I am looking for a more generic marker.  
> Thanks for any advise.
> 			Regards
> 			BJC - Brian	Short people... last to know its
> 					raining, first to know its a flood.

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