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Use of PVP in ELISA

C. Liu cliu at IC.NET
Mon Mar 20 09:45:35 EST 1995

>As suggested by Bioreba we use polyvinylpyrrolidone in extraction and 
>conjugate buffers when carrying out ELISA for detection of virus in 
>plant sap. I would be grateful if someone could enlighten me as to why 
> it is used . Is its action as a blocker or as an antioxidant or does it have 
>some other function?
>Isla Browning
I believe it's used as a blocking agent in your assay.  It is a hydrophilic 
polymer that is a wetting agent, and probably reduces the nonspecific 
binding to plastic in microtiter plates.
cliu at ic.net
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