<<Your message>>
>We would like to initiate some PCR based methods near Quito,
>Ecuador at an altitude of 3050 m above sea level. Water boils
>here at 89 C which will not allow us to bring the vials up above
>this temperature. Our PCR machine has not yet arrived but we
>would like to have some ideas on how this problem might be solved
>before it does.
>Many thanks
>Greg Forbes
<<End of your message>>
What an interesting problem! I somehow missed this when it was first posted.
My immediate thought is: would this still be a problem if you had one of those
thermal cyclers with a screw down heated lid? Also, if water boils at a lower
temperature, will the Tm of your DNA primers be lower as well?
Wish I knew more physics.
Kevin O'Donnell
Diagnostics and Molecular Biology
Scottish Agricultural Science Agency
Edinburgh odonnell at sasa.gov.uk
Kevin O'Donnell
Diagnostics and Molecular Biology
Scottish Agricultural Science Agency
Edinburgh odonnell at sasa.gov.uk
