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low boiling point

Wed Mar 22 23:10:37 EST 1995

>On 21 Mar 1995 WHEATCROFTR at ncccot.agr.ca wrote:

> Elevate the boiling point by adding DMSO

>Pardon me, but a think that using DMSO is not a good idea, since
>=B4=EF=BE=A0 p4D=C5&=D6 =E6=B6=ACc7=A7'D&$=EADMSO brakes
>hidrogen bonds and it interferes with the anealing process.DMSO is used in
>strand separation procedures.=20

>Return-path: <dsleal at academ01.mty.itesm.mx>
Dear Diana and other DMSO doubters,

See Shen et al, Trends in Genetics 8:227 (1992). Title: DMSO improves PCR...
I think they had 5% in their samples. This should push the BP up enough for 
Quito. [I believe the BP of neat DMSO is 189 C at sea level]

Roger W.

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