>On 21 Mar 1995 WHEATCROFTR at ncccot.agr.ca wrote:
> Elevate the boiling point by adding DMSO
>Pardon me, but a think that using DMSO is not a good idea, since
>=BFf=3D=A7=ACN=E9{=ABE=B4=E4x=AANx<=B7=FE=D2'=D9"=B7=CC=A6LMW}2y=E3=F0=EB=
>=B4=EF=BE=A0 p4D=C5&=D6 =E6=B6=ACc7=A7'D&$=EADMSO brakes
>hidrogen bonds and it interferes with the anealing process.DMSO is used in
>strand separation procedures.=20
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>Return-path: <dsleal at academ01.mty.itesm.mx>
Dear Diana and other DMSO doubters,
See Shen et al, Trends in Genetics 8:227 (1992). Title: DMSO improves PCR...
I think they had 5% in their samples. This should push the BP up enough for
Quito. [I believe the BP of neat DMSO is 189 C at sea level]
Roger W.
